肠道上皮氧化应激细胞模型的研究进展

doi:10.11843/j.issn.0366-6964.2014.03.001

Abstract

Abstract:

Intestinal epithelial cells are easily injured by oxidative stress in many ways.There are two methods to study intestinal epithelial cells injure：using animal model and/or cell model.But there are some disadvantages in using animal model only，such as high cost，data unsteadiness and difficult to study mechanism.So，to develop a suitable cell model for intestinal epithelial cells oxidative stress research is necessary.Now，the most oxidative stress inducing method used include physical method (such as radiation，anoxia/reoxygenation) and chemical process (such as add hydrogen peroxide or (hypo-)xanthine/xanthine oxidase).The every method has its own application condition，so the reseachers should select the most suitable one according to the research objectives.The most intestinal epithelial cells used can be classed into two main types：primary culture cells and subculture cells.The primary culture cells can reflect the characteristics of intestinal epithelial cells in vivo，but the intricacy and onerous operation limit their widely application.There are a lot types of subculture cells.They have different source and characteristics.The reseachers should select carefully.

CLC Number:

S852.21

CAI Xuan，WANG Jing-xian，CHEN Xiao-lian，GUO Qi，ZHU Li-hui，XU Jian-xiong. The Research Progress in Intestinal Epithelial Oxidative Stress Cell Model[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(3): 337-346.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2014.03.001

https://www.xmsyxb.com/EN/Y2014/V45/I3/337

References

［1］CAI X,CHEN X L,WANG X C，et al.Pre-protective effect of lipoic acid on injury induced by H₂O₂ in IPEC-J2 cells ［J］.Mol Cell Biochem，2013，378(1-2)：73-81.
［2］CENCI? A，LANGERHOLC T.Functional cell models of the gut and their applications in food microbiology—a review［J］.Int J Food Microbiol，2010，141：S4-S14.
［3］RILEY P A.Free radicals in biology：oxidative stress and the effects of ionizing radiation［J］.Int J Radiat Biol，1994，65(1)：27-33.
［4］MOLL M，PAN S J.Radiation-induced intestinal inflammation ［J］.World J Gastroentero，2007，13(22)：3043-3046.
［5］DATTA K,SUMAN S,KALLAKURY B V S，et al.Exposure to heavy ion radiation induces persistent oxidative stress in mouse intestine［J］.PLoS ONE，2012，7(8)：e42224.
［6］SAADA H N,REZK R G，ELTAHAWY N A.Lycopene protects the structure of the small intestine against gamma-radiation-induced oxidative stress［J］.Phytother Res，2010，24(Suppl.2)：S204-S208.
［7］李军,邱啸臣，王俊杰.N-乙酰半胱氨酸对辐照引起的大鼠肠隐窝上皮细胞损伤的减轻作用［J］.广东医学，2012，(15)：2203-2205.
［8］LYONS N M，O’BRIEN N M.Modulatory effects of an algal extract containing astaxanthin on UVA-irradiated cells in culture［J］.J Dermatol Sci，2002，30(1)：73-84.
［9］潘璠，刘成霞.谷氨酰胺对体外肠上皮细胞缺血再灌注损伤后occludin蛋白表达的影响［J］.中华临床医师杂志(电子版)，2012，(10)：2624-2627.
［10］WASA M,SOH H,SHIMIZU Y，et al.Glutamine stimulates amino acid transport during ischemia-reperfusion in human intestinal epithelial cells［J］.J Surg Res，2005，123(1)：75-81.
［11］PASZTI-GERE E,CSIBRIK-NEMETH E,SZEKER K，et al.Acute oxidative stress affects IL-8 and TNF-α expression in IPEC-J2 porcine epithelial cells［J］.Inflammation，2012,35(3):994-1004.
［12］赵保路.氧自由基和天然抗氧化剂［M］.北京：科学出版社，1999:290.
［13］ZHU L H,ZHAO K L,CHEN X L，et al.Impact of weaning and an antioxidant blend on intestinal barrier function and antioxidant status in pigs［J］.J Anim Sci，2012，90(8)：2581-2589.
［14］SHIN H S,ZHAO Z H,SATSU H，et al.Synergistic effect of tumor necrosis factor-alpha and hydrogen peroxide on the induction of IL-8 production in human intestinal Caco-2 cells［J］.Inflammation，2011，34(5)：440-447.
［15］王啸春.氧自由基对大鼠小肠上皮细胞的损伤及硫辛酸的干预研究［D］.上海：上海交通大学，2011.
［16］刘扬,池磊,冯琳，等.维生素C对建鲤肠上皮细胞氧化损伤保护作用的研究［J］.动物营养学报，2012，(8)：1503-1511.
［17］刘琳娜,张峰,梅其炳，等.唐古特大黄多糖对过氧化氢诱导的肠上皮细胞凋亡的保护作用及其可能机制［J］.中国药理学通报，2010，(7)：877-881.
［18］张芸,周文斌，刘萍.金丝桃苷抑制过氧化氢诱导IEC-6细胞凋亡的研究［C］.海口：中国化学会第9届天然有机化学学术会议，2012:1.
［19］TAKADA M,OTAKA M,TAKAHASHI T，et al.Overexpression of a 60-kDa heat shock protein enhances cytoprotective function of small intestinal epithelial cells［J］.Life Sci，2010，86(13-14)：499-504.
［20］WANG N,WANG G,HAO J X，et al.Curcumin ameliorates hydrogen peroxide-induced epithelial barrier disruption by upregulating heme oxygenase-1 expression in human intestinal epithelial cells［J］.Digest Dis Sci，2012，57(7)：1792-1801.
［21］IVISON S M,WANG C,HIMMEL M E，et al.Oxidative stress enhances IL-8 and inhibits CCL20 production from intestinal epithelial cells in response to bacterial flagellin［J］.Am J Physiol-Gastr L，2010，299(3)：G733-G741.
［22］PICCOLOMINI A F,ISKANDAR M M,LANDS L C，et al.High hydrostatic pressure pre-treatment of whey proteins enhances whey protein hydrolysate inhibition of oxidative stress and IL-8 secretion in intestinal epithelial cells［J］.Food Nutr Res，2012，56：1-10.
［23］YOUNG D,FAN M Z，MINE Y.Egg yolk peptides up-regulate glutathione synthesis and antioxidant enzyme activities in a porcine model of intestinal oxidative stress［J］.J Agr Food Chem，2010，58(13)：7624-7633.
［24］程潇钰.胰高血糖素样肽-2对原代培养大鼠小肠黏膜上皮细胞的作用研究［D］.大连：大连医科大学，2010.
［25］GU B H,MINH N V,LEE S H，et al.Deoxyschisandrin inhibits H₂O₂-induced apoptotic cell death in intestinal epithelial cells through nuclear factor-kappa B［J］.Int J Mol Med，2010，26(3)：401-406.
［26］王境岩,朱圣庚，徐长法.生物化学［M］ .第3版.北京：高等教育出版社，2002:681.
［27］海春旭.自由基医学［M］ .西安：第四军医大学出版社，2006.
［28］庄祥云.在炎症性肠道疾病中氧化应激反应的作用［J］.日本医学介绍，1995，(12)：566-567.
［29］SASAKI M，JOH T.Oxidative stress and ischemia-reperfusion injury in gastrointestinal tract and antioxidant，protective agents ［J］.J Clin Biochem Nutr，2007，40(1)：1.
［30］CHEN Q,ZHANG S M,LIU H Y，et al.Protective effect of glutathione against oxidative injury in intestinal epithelial cells of piglet in vitro ［J］.Anim Biol，2009，59(3)：263-272.
［31］MUKOJIMA K,MISHIMA S,ODA J，et al.Protective effects of free radical scavenger edaravone against xanthine oxidase-mediated permeability increases in human intestinal epithelial cell monolayer［J］.J Burn Care Res，2009，30(2)：335-340.
［32］杨家军.早期断奶仔猪肠上皮细胞氧化与损伤及二氢杨梅素对其调控作用［D］.北京：中国农业科学院，2012.
［33］RAO R,BAKER R D，BAKER S S.Inhibition of oxidant-induced barrier disruption and protein tyrosine phosphorylation in Caco-2 cell monolayers by epidermal growth factor［J］.Biochem Pharmacol，1999，57(6)：685-695.
［34］RAO R,LI L,BAKER R D，et al.Glutathione oxidation and PTPase inhibition by hydrogen peroxide in Caco-2 cell monolayer［J］.Am J Physiol-Gastr L，2000，279(2)：G332-G340.
［35］CIOFFI G,PESCA M S,DE CAPRARIIS P，et al.Phenolic compounds in olive oil and olive pomace from Cilento (Campania，Italy) and their antioxidant activity［J］.Food Chem，2010，121(1)：105-111.
［36］陈群,于维,于秀芳，等.氧化应激对离体大鼠小肠上皮细胞的损伤及抗氧化研究［J］.河北农业大学学报，2009，(6)：101-104.
［37］OHKAWARA T,TAKEDA H,NISHIWAKI M，et al.Protective effects of heat shock protein 70 induced by geranylgeranylacetone on oxidative injury in rat intestinal epithelial cells［J］.Scand J Gastroentero，2006，41(3)：312-317.
［38］PARK S C,LIM J Y,JEEN Y T，et al.Ethanol-induced DNA damage and repair-related molecules in human intestinal epithelial Caco-2 cells［J］.Mol Med Rep，2012，5(4)：1027-1032.
［39］司徒镇强，吴军正.细胞培养［M］ .第2版.西安：世界图书出版社，2007:10-11,57.
［40］BOOTH C,O'SHEA J A，POTTEN C S.Maintenance of functional stem cells in isolated and cultured adult intestinal epithelium［J］.Exp Cell Res，1999，249(2)：359-366.
［41］王静,张彦明,仝钢，等.新生仔猪小肠上皮细胞的分离培养和鉴定［J］.畜牧兽医学报，2010，41(1)：92-98.
［42］李莉,高淑静,张守全，等.新生仔猪小肠上皮细胞体外培养的研究［J］.仲恺农业工程学院学报，2010，23(1):34-37.
［43］HOEY D E E,SHARP L,CURRIE C，et al.Verotoxin 1 binding to intestinal crypt epithelial cells results in localization to lysosomes and abrogation of toxicity［J］.Cell Microbiol，2003，5(2)：85-97.
［44］DZIVA F,MAHAJAN A,CAMERON P，et al.EspP，a Type V-secreted serine protease of enterohaemorrhagic Escherichia coli O157:H7，influences intestinal colonization of calves and adherence to bovine primary intestinal epithelial cells［J］.Fems Microbiol Lett，2007，271(2)：258-264.
［45］戴定威,吴圣楣,李敏，等.新生大鼠小肠上皮细胞分离培养研究［J］.细胞生物学杂志，1997，1：31-35.
［46］CHOPRA D P,DOMBKOWSKI A A,STEMMER P M，et al.Intestinal epithelial cells in vitro ［J］.Stem Cells Dev，2010，19(1)：131-142.
［47］GON ALVES P，GREG RIO I，CATARINO T A，et al.The effect of oxidative stress upon the intestinal epithelial uptake of butyrate［J］.Eur J Pharmacol，2013，699(1-3)：88-100.
［48］KARRASCH T,SPAETH T,ALLARD B，et al.PI3K-Dependent GSK3β(Ser9)-phosphorylation is implicated in the intestinal epithelial cell wound-healing response［J］.PLoS ONE，2011，6(10)：e26340.
［49］GARC A-NEBOT M J,BARBER R，ALEGR A A.Iron and zinc bioavailability in Caco-2 cells：Influence of caseinophosphopeptides［J］.Food Chem，2013，138(2-3)：1298-1303.
［50］KLUXEN F,DIEL P,H FER N，et al.The metallohormone cadmium modulates AhR-associated gene expression in the small intestine of rats similar to ethinyl-estradiol［J］.Arch Toxicol，2013，87(4)：633-643.
［51］WANG X H,TANG S,LE S Y，et al.Aberrant expression of oncogenic and tumor-suppressive MicroRNAs in cervical cancer is required for cancer cell growth［J］.PLoS ONE，2008，3(7)：e2557.
［52］DYDENSBORG A B,HERRING E,AUCLAIR J，et al.Normalizing genes for quantitative RT-PCR in differentiating human intestinal epithelial cells and adenocarcinomas of the colon［J］.Am J Physiol-Gastr L，2006，290(5)：G1067-G1074.
［53］潘阳林,毕锋,刘娜，等.Rho家族蛋白在胃癌细胞系中的表达及意义［J］.解放军医学杂志，2003，(6)：517-519.
［54］LIU C T,CHU F J,CHOU C C，et al.Antiproliferative and anticytotoxic effects of cell fractions and exopolysaccharides from Lactobacillus casei［J］.Mutat Res-Gen Tox En，2011，721(2)：157-162.
［55］陈平.赭曲霉毒素A对IPEC-J2细胞Nrf2抗氧化系统的影响及硒的保护效应研究［D］.雅安：四川农业大学，2010.
［56］PARTHASARATHY G，MANSFIELD L S.Recombinant interleukin-4 enhances Campylobacter jejuni invasion of intestinal pig epithelial cells (IPEC-1)［J］.Microb Pathogenesis，2009，47(1)：38-46.
［57］曾黎明.精氨酸对猪肠道上皮细胞IPEC-1精氨酸转运、细胞增殖及基因表达水平影响的研究［D］.长沙：湖南农业大学，2012.
［58］CHINGWARU W,MAJINDA R T,YEBOAH S O,et al.Tylosema esculentum (Marama) tuber and bean extracts are strong antiviral agents against rotavirus infection［J］.Evid-Based Compl Alt，2011，8(1):1.
［59］QUARONI A,WANDS J,TRELSTAD R L，et al.Epithelioid cell cultures from rat small intestine.Characterization by morphologic and immunologic criteria［J］.J Cell Biol，1979，80(2)：248-265.
［60］陈祥贵,芮光伟,任川洪，等.IEC-6细胞的培养鉴定和生长特性研究［J］.四川工业学院学报，2003，22(1)：4.
［61］谢艳兰,郭昊蔚,郑羡慕，等.Caco-2细胞模型在茶叶生物活性成分研究中的应用［J］.茶叶科学，2012，(6)：471-479.
［62］余自成,高瑜,田薇薇，等.肠道转运Caco-2细胞单层模型的建立及验证评价［J］.中国临床药学杂志，2012，(04)：218-221.
［63］SUN H,CHOW E C,LIU S，et al.The Caco-2 cell monolayer：usefulness and limitations［J］.Expert Opin Drug Metab Toxicol，2008，4(4):395-411.
［64］BROSNAHAN A J，BROWN D R.Porcine IPEC-J2 intestinal epithelial cells in microbiological investigations［J］.Vet Microbiol，2011，156：229-237.
［65］MCORIST S,JASNI S,MACKIE R A，et al.Entry of the bacterium ileal symbiont intracellularis into cultured enterocytes and its subsequent release［J］.Res Vet Sci，1995，59(3)：255-260.
［66］ZHU J,YIN X,YU H，et al.Involvement of quorum sensing and heat-stable enterotoxin a in cell damage caused by a porcine enterotoxigenic Escherichia coli strain［J］.Infect Immun，2011，79(4)：1688.
［67］LUNNEY J K.Advances in swine biomedical model genomics［J］.Int J Biol Sci，2007，3(3)：179.
［68］GEENS M，NIEWOLD T.Optimizing culture conditions of a porcine epithelial cell line IPEC-J2 through a histological and physiological characterization［J］.Cytotechnology，2011，63(4)：415-423.
［69］LANGERHOLC T,MARAGKOUDAKIS P A,WOLLGAST J，et al.Novel and established intestinal cell line models-an indispensable tool in food science and nutrition［J］.Trends Food Sci Technol，2011，20：S11-S20.

[1]	LIU Jie, CONG Wei, ZHAO Mindie, ZHAO Ruqian. Expression of GR and FKBP5 in Hippocampus and Hypothalamus of Arbor Acres and Rugao Yellow Broiler Chickens and Its Relationship with Stress Sensitivity [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4766-4776.
[2]	YANG Heng, LI Licai, FU Lin, XU Huihao, ZHANG Dezhi, LI Qianyong. Effect of Prostaglandin D₂ on Endocrine Function and Expression of Apoptosis-related Genes in Goat Luteal Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1652-1663.
[3]	YE Qianwen, CHEN Zhuo, LI Xin, SUN Yawei, JIN Xiaoye, LI Ziqian, WUMAIERJIANG·Yahefu, ZHONG Qi, MA Xuelian, YAO Gang. Dynamic Changes of Gut Microbiota Composition and Its Predicted Metabolism Function in One-month-old Sucking Lambs [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1095-1108.
[4]	LIU Zhihao, JIA Peng, LAI Qi, DONG Lifeng, WU Qiujue, GAO Yanhua, TIAN Zhonghong, DIAO Qiyu. Characteristics of Rumen Fermentation and Methane Production in Different Parity Dry Cows [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4296-4305.
[5]	YUAN Jing, ZHANG Xinxin, ZHOU Di, YANG Shuai, ZHOU Jiayan, LIANG Aixin, YANG Liguo. Evaluation of the Effect of SS and CST Dual-expression DNA Vaccines with Attenuated Salmonella as Carrier in Immunizing Calves through Different Routes [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(6): 1914-1924.
[6]	MA Tianying, LI Yilin, DONG Xinyue, SI Rongrong, BAI Xue, WANG Zixu, CHEN Yaoxing, CAO Jing. Food Intake and Hypothalamic Energy Balance [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(7): 1789-1799.
[7]	ZHANG Chuntao, MA Tao, TU Yan, DIAO Qiyu. The Relationship between Biorhythms and Physiological Nutrients and Substance Digestion and Utilization in Animals [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(4): 872-880.
[8]	LI Fan, CHEN Xiaoyang, YANG Guoyu, LI Fuchang. The Regulatory Role of Wnt/β-catenin Signaling Pathway in the Development of Mammalian Hair Follicles [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(11): 2186-2194.
[9]	ZHANG Shilei, BAO Jialu, SHI Wanyu, ZHONG Xiuhui. Effects of Maternal Exposure to Bisphenol A during Pregnancy and Lactation on the Development of Testis in Male Offspring Mice [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(5): 993-1005.
[10]	MENG Fan-yong, DENG Yi-feng, YANG Jun-hua, CUI Jun, ZHOU Zhen-lei, HOU Jia-fa. Effects of TRPV6 Gene Silencing on the Expression of Proteins Involved in Calcium Ions Transcellular Transport in Duodenum and Jejunum of Laying Hens [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(11): 2505-2513.
[11]	SHEN Xiang-hua, PAN Deng, FAN Kui-kui, LI Qiang, LI Ting, DU Chen-guang. Ghrelin Mediate Female Mice Food Intake via Affecting on Estrogen Receptor-alpha Neuron in Arcuate Nucleus [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(12): 2399-2406.
[12]	LIANG Hong-wei，FENG Bo，ZHU Wen-yu，WANG Jian-fang，ZHANG Tao，MU Xiang. Cultivation and Purification of Rat Pulmonary Microvascular Endothelial Cells in vitro [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(10): 2143-2150.
[13]	BAI Hao，SUN Yan-yan，CHEN Ji-lan. The Research Progress of Beak Deformity [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(3): 417-422.

The Research Progress in Intestinal Epithelial Oxidative Stress Cell Model

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 13

Recommended Articles

Metrics

Comments